Spermine Tetrahydrochloride for Research

Spermine Tetrahydrochloride is the hydrochloride salt of spermine, a naturally occurring polyamine involved in cellular biochemical processes. With the molecular formula C10H30Cl4N4 and a molecular weight of 348.18 g/mol, it is used primarily in biochemical research, molecular biology, and laboratory studies requiring a defined polyamine reagent with documented identity and purity.

Product Overview

Spermine Tetrahydrochloride is a water-soluble salt form of spermine, a polyamine containing four nitrogen atoms and multiple protonatable amino groups. Its positively charged character under many aqueous conditions makes it relevant to research involving nucleic acids, membranes, macromolecular interactions, and cellular biochemistry.

For research laboratories and biotechnology procurement teams, product selection should focus on chemical identity, assay basis, water content, solubility, and compatibility with the experimental system. Because polyamine reagents can influence nucleic-acid behavior and other biochemical interactions, consistent specifications and suitable storage are important for reproducible research.

What Is Spermine Tetrahydrochloride?

Spermine Tetrahydrochloride is the tetrahydrochloride salt of spermine, a naturally occurring aliphatic polyamine found in biological systems. The hydrochloride form provides a defined salt form for laboratory preparation and biochemical investigation. Its four nitrogen-containing sites contribute to its polycationic behavior under suitable solution conditions.

The compound is commonly used in molecular biology and biochemical research, including investigations of nucleic-acid structure, macromolecular interactions, and cell-free experimental systems. Research use does not establish suitability for human consumption, clinical use, or any specific therapeutic application.

Specifications of Spermine Tetrahydrochloride

Key Characteristics and Research Value

• Defined salt form: The tetrahydrochloride form provides a specified counterion composition for reagent preparation and analytical identification.
• Polyamine chemistry: Spermine contains multiple protonatable amino groups and can interact electrostatically with negatively charged biological macromolecules under appropriate conditions.
• Aqueous preparation: Its water solubility supports preparation of aqueous stock solutions for compatible laboratory protocols.
• Biochemical research relevance: The compound is used in investigations involving nucleic acids, macromolecular organization, and polyamine-related biochemical processes.
• Specification-based procurement: Assay, water content, impurity profile, and documentation can be assessed against the needs of the intended research application.

Applications of Spermine Tetrahydrochloride

Molecular Biology and Nucleic-Acid Research

Spermine Tetrahydrochloride is used in laboratory investigations of DNA and RNA interactions, nucleic-acid conformation, and the behavior of negatively charged biomolecules. Experimental concentrations and solution conditions should be selected for the specific protocol because polyamine effects depend on ionic strength, pH, and the composition of the reaction mixture.

Biochemical and Cell-Free Research

The compound may be used as a defined polyamine reagent in biochemical assays and cell-free experimental systems. Researchers should validate concentration ranges and reagent compatibility for each assay rather than assume that a concentration suitable for one system will produce the same outcome in another.

Macromolecular Interaction Studies

Its polycationic characteristics make spermine relevant to research on electrostatic interactions involving nucleic acids and other negatively charged macromolecules. The choice of salt form, buffer composition, and concentration can affect experimental interpretation and should be documented for reproducibility.

Laboratory Reagent Preparation

Spermine Tetrahydrochloride can be used to prepare research solutions when the selected grade, concentration, and handling procedure meet the laboratory's requirements. Prepared solutions should be managed according to the validated protocol, including any applicable storage, stability, and contamination-control requirements.

Buyer-Decision Guide: Salt Form, Assay Basis, and Solution Preparation

For Spermine Tetrahydrochloride, a key purchasing consideration is the distinction between the tetrahydrochloride salt and free-base spermine. These forms are not interchangeable on a mass-for-mass basis. The hydrochloride salt has a higher molecular weight because of its associated hydrochloride groups, so weighing the same mass of each form does not provide the same molar quantity of spermine.

1. Confirm the Salt Form

Specify Spermine Tetrahydrochloride, CAS 306-67-2, when the protocol requires the tetrahydrochloride salt. Do not substitute spermine free base or another salt without recalculating the required molar quantity and checking compatibility with the experimental method.

2. Calculate the Required Molar Quantity

Using a molecular weight of 348.18 g/mol, the theoretical mass for a 10 mmol quantity of Spermine Tetrahydrochloride is approximately 3.48 g before any adjustment for assay or water content. For actual preparation, follow the batch-specific assay basis and correct the weighed amount where required by the validated protocol.

3. Compare Purity Results on the Same Basis

4. Match Packaging to Laboratory Consumption

Small containers may be practical for low-throughput analytical work because they reduce repeated opening of the full supply. Larger packages may suit laboratories with predictable consumption and established handling procedures. Select packaging based on usage rate, moisture protection, storage capacity, and the supplier's stability documentation.

Why Choose This Spermine Tetrahydrochloride Specification

• Clear compound identification: Defined CAS number, molecular formula, and molecular weight support purchasing accuracy.
• Research-grade specification options: Purity targets can be selected according to assay sensitivity, experimental requirements, and budget.
• Batch-level quality documentation: COA, TDS, and SDS documents support incoming inspection and laboratory reagent management.
• Flexible supply arrangements: Research quantities and larger orders can be discussed according to packaging, documentation, and transport needs.
• Analytical verification: Appropriate identity and purity methods help buyers assess material suitability before use in critical experiments.

Packaging and Bulk Supply

Packaging options can support laboratory evaluation, ongoing research use, and larger qualified orders. Small research containers are useful for controlled dispensing, while moisture-barrier packaging can support larger quantities when appropriate for the material and shipping conditions.

Minimum order quantity and lead time depend on the selected grade, stock or production availability, packaging format, and documentation requirements. Buyers should specify their target purity, quantity, and intended research use when requesting a quotation.

OEM and Customization Support

For suitable research supply projects, product requirements may include assay targets, water-content limits, impurity criteria, packaging size, labeling, and analytical documentation. Custom specifications should be documented and agreed before order placement.

Any proposed use in foods, dietary supplements, cosmetics, or pharmaceutical products requires separate assessment of ingredient legality, safety, quality standards, and applicable market regulations. Availability as a laboratory reagent does not establish approval or suitability for human consumption.

Quality Control and Documentation

A fit-for-purpose quality plan should address the attributes relevant to the salt form and intended research application. Depending on the agreed specification, quality review may include:

• Identity verification using an appropriate instrumental or spectroscopic method.
• Assay or purity testing using a suitable validated analytical method.
• Water-content determination where relevant to accurate reagent preparation.
• Related-substance evaluation against agreed acceptance criteria.
• Residual-solvent testing where indicated by the manufacturing process and risk assessment.
• Elemental-impurity testing where required by the intended use or specification.
• Batch traceability, packaging integrity, storage instructions, and expiry or retest-period review.

Microbiological testing should be included when relevant to the intended application and its quality requirements. Numerical acceptance limits and batch results should be based on the agreed specification and supporting test documentation rather than assumed in advance.

Frequently Asked Questions

What is the molecular weight of Spermine Tetrahydrochloride?

Spermine Tetrahydrochloride has a molecular weight of approximately 348.18 g/mol and the molecular formula C10H30Cl4N4. These values refer to the tetrahydrochloride salt, not spermine free base. Confirming the salt form is important when calculating molar quantities for buffers, biochemical assays, or other laboratory preparations.

Is Spermine Tetrahydrochloride water-soluble?

Spermine Tetrahydrochloride is water-soluble and is used to prepare aqueous solutions for compatible laboratory applications. The concentration that can be used in a particular experiment depends on the buffer, pH, ionic strength, temperature, and other components. Validate solution preparation and handling conditions for the intended protocol.

What purity should I choose?

The appropriate purity depends on the sensitivity of the experiment and the required impurity profile. Common research-grade specifications may include 95% and 98% or higher, but available grades vary. Review the assay method, water content, identity data, and batch-specific COA rather than relying on the percentage alone.

What documents should accompany the order?

Request a batch-specific certificate of analysis, technical data sheet, and safety data sheet. For sensitive biochemical work, also review the assay basis, water-content result where relevant, and identity confirmation. The documents should identify Spermine Tetrahydrochloride specifically and match the batch being supplied.

Conclusion

Spermine Tetrahydrochloride is a specialized polyamine reagent for molecular biology and biochemical research. Successful procurement depends on confirming the hydrochloride salt form, selecting an appropriate assay specification, reviewing water content where relevant, and matching storage and packaging to laboratory use. Clear batch documentation helps support accurate preparation and reproducible experimental work.

Contact us today to request samples, a COA, technical documents, and a wholesale quotation for Spermine Tetrahydrochloride.