Spermine Dihydrate for Biochemical Research

Spermine Dihydrate for Biochemical Research

Spermine dihydrate is a hydrated form of spermine, a naturally occurring polyamine used in biochemical and molecular biology research. With the molecular formula C10H30N4O2 and a molecular weight of 238.37 g/mol, it is relevant to laboratory studies of nucleic acids, ribosomes, and macromolecular interactions where the specified hydrate form and accurate molar calculations are important.

For research reagent procurement, spermine dihydrate should be evaluated according to chemical identity, assay purity, water content, analytical method, and batch documentation. Its hydrate state distinguishes it from anhydrous spermine and spermine hydrochloride salts, making the exact chemical form essential for reproducible laboratory preparation.

What Is Spermine Dihydrate?

Spermine is a tetraamine belonging to the polyamine family. It occurs naturally in biological systems and participates in interactions with negatively charged biomolecules, including DNA and RNA. Spermine dihydrate consists of spermine associated with two water molecules per formula unit.

The hydrate form has a theoretical molecular formula of C10H30N4O2, commonly written as C10H26N4·2H2O. Its molecular weight is approximately 238.37 g/mol. The CAS number for spermine dihydrate is 403982-64-9, distinguishing it from anhydrous spermine, which has CAS number 71-44-3.

Spermine dihydrate is used as a research reagent in biochemical studies and selected molecular biology applications. Its suitability depends on the experimental protocol, required purity, and verified characteristics of the supplied material.

Spermine Dihydrate Specifications

Key Characteristics of Spermine Dihydrate

• Defined chemical identity: The dihydrate form has a distinct CAS number and molecular weight, supporting accurate identification and procurement.
• Polyamine structure: Spermine contains multiple amino groups that contribute to its interactions with negatively charged biomolecules.
• Research relevance: Spermine is used in studies involving nucleic acids, ribosomes, and biochemical macromolecular interactions.
• Water-soluble properties: Its aqueous solubility makes it suitable for selected laboratory solution-preparation procedures.
• Specification-based quality assessment: Assay results, water content, identity testing, and batch traceability support incoming material evaluation.

Applications of Spermine Dihydrate

Molecular Biology and Nucleic Acid Research

Spermine-based reagents are used in selected studies of DNA and RNA structure, nucleic acid interactions, and the behavior of charged biomolecules. When a protocol specifies spermine dihydrate, researchers should verify that the material's chemical form and assay basis match the method requirements.

Biochemistry and Ribosome Research

Spermine is relevant to investigations of ribosomal systems, cellular macromolecules, and polyamine-associated biochemical processes. Research applications should be selected according to the experimental design, required reagent grade, and analytical acceptance criteria.

Laboratory Reagent Preparation

Spermine dihydrate can be incorporated into research solutions and laboratory reagent preparations when specified by the experimental method. Calculations should account for the dihydrate molecular weight, the reported assay basis, and the required final concentration.

Biochemical Formulation Research

Researchers evaluating polyamine-containing formulations should consider the exact chemical form, solution conditions, and compatibility with other formulation components. Product selection should be based on the requirements of the intended experiment rather than on the product name alone.

Buyer Selection Guide: Hydrate Form, Purity, and Assay Method

The defining procurement consideration for spermine dihydrate is the relationship between its hydrate state and the calculation of molar concentration. Although spermine dihydrate and anhydrous spermine contain the same parent polyamine, their molecular weights differ because the dihydrate includes two water molecules per formula unit. This distinction affects the mass required to prepare a specified molar concentration.

Purity selection: Choose a purity grade that meets the experimental method's acceptance criteria. A higher assay value does not eliminate the need to confirm chemical identity, water content, and the reporting basis used by the analytical method.

Analytical method selection: Review the COA to determine whether assay is reported by GC, HPLC, titration, or another suitable method. Water content may require a separate measurement, such as Karl Fischer titration. Different methods measure different properties, so results should not be compared without considering their analytical basis.

Calculation example: A theoretical 1 mmol quantity of spermine dihydrate corresponds to approximately 238.37 mg before any correction for assay purity. The corresponding theoretical mass for 1 mmol of anhydrous spermine is approximately 202.34 mg. Actual weighing calculations should follow the product's stated assay basis and the laboratory's validated procedure.

Why Choose This Spermine Dihydrate Product

• Identity-led sourcing: Procurement can be aligned with the specified CAS number, hydrate form, assay requirements, and intended research application.
• Batch documentation: COA, TDS, and SDS documentation supports technical review and laboratory purchasing procedures.
• Relevant analytical testing: Identity, assay, and water-content requirements can be defined according to the product specification and intended use.
• Flexible packaging: Laboratory quantities, bulk orders, and customized pack formats can be arranged according to procurement needs.
• Technical specification review: Confirming the formula, molecular weight, and assay basis before purchase helps reduce avoidable errors in reagent preparation.

Packaging and Bulk Supply

Spermine dihydrate can be supplied in laboratory-scale packs and larger quantities according to the selected grade and order requirements. Sealed containers and moisture-protective packaging are important considerations for preserving the specified material characteristics during storage and transport.

For bulk procurement, buyers should specify the required chemical identity, purity grade, assay method, water-content criteria, pack size, order quantity, and documentation requirements. Minimum order quantities and packaging configurations depend on the selected grade and supply format.

OEM, Private Label, and Customized Packaging

For eligible commercial projects, customized packaging, labeling, and order configurations can be discussed according to the product specification and target market. Labels and technical documents should accurately reflect the verified chemical identity, molecular formula, hydrate state, and applicable safety information.

Quality Control and Documentation

Quality control should be based on the agreed specification for the exact product supplied. Relevant checks may include:

• Identity verification using an appropriate analytical method.
• Assay or purity testing using a suitable validated method.
• Water-content assessment where required by the hydrate specification.
• Review of applicable impurity limits and product-specific analytical requirements.
• Packaging integrity and batch traceability checks.
• Review of the COA, TDS, and SDS for consistency with the ordered material.

Analytical methods and acceptance limits should be appropriate for the intended research application. Sterility, endotoxin, residual-solvent, and microbiological specifications should only be assumed when explicitly defined and supported by the relevant product documentation.

Frequently Asked Questions

What is the CAS number of spermine dihydrate?

The CAS number for spermine dihydrate is 403982-64-9. Anhydrous spermine has a different CAS number, 71-44-3. Buyers should confirm the chemical name, molecular formula, and CAS number together when checking a quotation, product specification, or batch COA.

What is the molecular weight of spermine dihydrate?

Spermine dihydrate has a molecular weight of approximately 238.37 g/mol based on the formula C10H26N4·2H2O. Anhydrous spermine has a molecular weight of approximately 202.34 g/mol. The appropriate value must be used when converting between mass and molar quantity for laboratory preparations.

Which purity grade should I choose?

Select the grade that meets the analytical acceptance criteria of the intended research method. Review the reported assay value, test method, hydrate identity, and water-content requirements on the applicable product documentation. For sensitive experiments, define acceptance criteria before procurement rather than comparing products on assay percentage alone.

How should spermine dihydrate be stored?

Keep the material in a tightly closed, compatible container and follow the storage temperature and moisture precautions stated in the product-specific SDS and technical documentation. Protect the packaging from damage and unnecessary environmental exposure. The applicable supplier stability information should determine the storage conditions and shelf life for the purchased grade.

Conclusion

Spermine dihydrate is a defined hydrated polyamine reagent for biochemical and molecular biology research. Accurate sourcing depends on confirming its CAS number, molecular formula, molecular weight, assay purity, water-content requirements, and batch documentation. Contact us today to request samples, a COA, technical documents, and a wholesale quotation for spermine dihydrate.